Receptor-mediated activation of a phospholipase A2 in rabbit neutrophil plasma membrane.
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چکیده
منابع مشابه
Secretory phospholipase A2 receptor-mediated activation of cytosolic phospholipase A2 in murine bone marrow-derived mast cells.
The current study examined the signal transduction steps involved in the selective release of arachidonic acid (AA) induced by the addition of secretory phospholipase A2 (sPLA2) isotypes to bone marrow-derived mast cells (BMMC). Overexpression of sPLA2 receptors caused a marked increase in AA and PGD2 release after stimulation of BMMC, implicating sPLA2 receptors in this process. The hypothesis...
متن کاملPhospholipase A2 inhibition results in sequestration of plasma membrane into electronlucent vesicles during IgG-mediated phagocytosis.
Arachidonic acid is essential for antibody-mediated phagocytosis but its role in this process has not been defined. The phospholipase A2 inhibitor bromoenol lactone decreases arachidonic acid release and arrests phagocytosis; this effect is bypassed by the addition of arachidonic acid to bromoenol lactone-treated cells. In this morphological study, monocytes treated with bromoenol lactone accum...
متن کاملReceptor-mediated activation of phospholipase A2 and arachidonic acid release in signal transduction.
Neurotransmitters, hormones, cytokines, growth factors and other ligands transmit their specific messages by binding to receptor molecules on the cell surface. The ligand-bound receptors activate effector systems such as adenylate cyclase, cyclic nueleotide phosphodiesterase, ion channels, and phospholipases via membrane-associated transducing systems to generate second messengers. Receptors ac...
متن کاملA 2 receptor - mediated activation of cytosolic phospholipase
1. Abstract 2. Introduction 3. Materials and methods 3.1. Exogenous PLA2s 3.2. ExPLI 3.3. Cell culture 3.4. Determination of basement membrane invasiveness 3.5. Determination of cell chemotaxis 3.6. Determination of MMP activity (Zymography) 3.7. Determination of cell PLA2 activity 3.8. Determination of exogenous PLA2 activity 3.9. Identification of cell sPLA2 and sPLA2 receptor expression 3.10...
متن کاملEffects of divalent cations on prostaglandin biosynthesis and phospholipase A2 activation in rabbit kidney medulla slices.
The divalent cations Ca2+, Mg2+, Co2+, Mn2+, Sr2+, and Ba2+ were compared for their stimulatory or inhibitory effect on PG formation in rabbit kidney medulla slices. Calcium, manganese, and strontium ions stimulated PG generation up to 3- to 5-fold in a time- and dose-dependent manner (Ca2+ greater than Mn2+ approximately Sr2+) while the magnesium and cobalt ions were without significant effect...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1984
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.81.3.767